Hi!! Do anyone know if CD8+ T cells are affected by CpG in a culture? Because I want to coculture B cells and CD8+T cells, so I have to activate B cells with CpG; but what about CD8+T cells and CpG?
Thanks!!
Dear Yamila Gazzoni Yes, CpG does effect the clonal expansion of CD8 cytotoxic T cells. I hope the publication attached will be helpful in answering your query as the stimulants used in B cell culture (B Reg differentiation).
Dear Yamila!
Please You also look at the following article:
Article Poly-guanosine motifs costimulate antigen-reactive CD8 T cel...
In fact, independently of CpG-motifs, poly-G-ODN displayed the ability to costimulate T cells. Costimulation was operative on CD8 T cells but not CD4 T cells. Poly-G-mediated costimulation resulted in IL-2-driven T-cell proliferation and induced cytolytic T cells. Overall the data imply that poly-G motifs costimulate antigen reactive CD8 T cells, while CpG-DNA motifs fail to do so but may affect T-cell activation via APC derived cytokines such as IFN type I.
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