Yamila Gazzoni

3 Questions 9 Answers 0 Followers

Questions related from Yamila Gazzoni

How could increase survival of CD8+T cells?

Hi! I am culturing CD8+T cells from the spleen of infected mice, but they die a lot after ~16 hours. Any recommendations (such as cytokines) to increase their survival? IL-2 is not increasing...

20 August 2021 6,040 3 View

Are CD8+ T cells affected by CpG?

Hi!! Do anyone know if CD8+ T cells are affected by CpG in a culture? Because I want to coculture B cells and CD8+T cells, so I have to activate B cells with CpG; but what about CD8+T cells and...

12 April 2021 9,813 3 View

Is CXCR5 on T cells affected after a cell sorting?

I have tried to sort CD8+ CXCR5+ in the ARIA but the purity of my sample is about 50%. After sorting, I stain my samples to verify purity but it is low. So, I thought about "downregulation" or...

16 February 2021 8,383 7 View