What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

I.P. drug injection- time to reach brain?

I am injecting different serotonergic drugs into mice i.p. and see an effect in the brain electrophysiology quite fast, i.e. within minutes, when using a specific receptor agonist drug. I do not...

01 February 2021 8,406 3 View

When testing for outliers in a moderation analysis with a dichotomous moderator, is the overall sample size used to adjust Cook's and S.residuals?

This managed to throw me for a loop. When I'm assessing the presence of outliers before I conduct my moderation analysis I use SPSS to split the data file based upon the dichotomous moderator. I...

24 August 2020 6,578 3 View

Hello, does anyone have any links/resources for an extraction method of melatonin dissolved in AQ solution?

Hello, I currently have a product that has a small amount of melatonin dissolved in it (aqueous solution), and I need a sufficient amount to get a reading on an IR spec. I was thinking of...

04 December 2018 7,975 3 View

Does anyone have a protocol for the extraction of nuclear and cytoplasmic RNA from plant leaf tissue?

Hello, I'm looking for a protocol that will allow the extraction of nuclear and cytoplasmic RNA from the same plant leaf tissue sample (Arabidopsis thaliana). I have found protocols for the...

16 August 2018 158 2 View

Other than HPLC, is there a good method to measure intracellular retinoic acid (RA) concentrations in the cytosol of cultured cells?

I have come across an ELISA to measure RA levels in serum/plasma, however I am not sure if this sensitive enough to determine RA concentrations in cell lysates (0.625-10 ng/mL). I was hoping...

10 October 2017 2,650 2 View

Does anyone know a good way of getting plasma from frozen whole blood?

11 July 2017 9,233 4 View

How to prevent background DIG staining in in situ PCR method?

I am performing in situ PCR of an RNA target in bovine lymph tissue; incorporating the DIG-dUTPs directly in the PCR product and then detection via anti-DIG Fab AP binding and subsequent...

08 February 2016 5,716 3 View

Are item means typically different in Xcalibre?

I am running an IRT analysis on an instrument in XCalibre, and the analysis reports substantially different means for the items than those calculated in Excel? Is there some weighting happening of...

30 January 2015 7,946 3 View

Is there a way to use immunohistochemistry to show changes in metabolism of murine muscle tissue following schema (glucose vs lipids etc)?

I am using the hind limb ischemia model in NOD/SCID mice and would like to measure changes in metabolism to the localised tissue both in a control mouse and mouse with recovered hind limb...

02 July 2014 7,096 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Is it possible to conduct a simple linear regression with a continuous value as an DV and 3 categorical ones for the IV?

I am currently writing my dissertation on the potential effect of bilingualism on intelligence scores. The latter is represented by a scale on the Y axe. My independent variable is the linguistic...

24 January 2021 5,336 2 View

What regression model should we use?

Our research will focus on 5 countries and the timeline will cover 5 years. We have to IV, 8 DVs, and some moderating variables. Initially, we will use panel regression analysis but we do not know...

09 January 2021 8,962 3 View

Need help in Andrew F. Hayes model of moderation?

I have one IV(scale) and two DV(Scale) and my moderator is a dichotomous variable. How to use Andrew F. Hayes process in SPSS for this analysis and reporting in APA style.

25 December 2020 1,500 5 View

How can we confirm the formation of heterojunction in nanocomposite systems ?

IV measurement and KPFM

21 December 2020 9,658 4 View

How to calculate the flow rate required for fluidization for a fluidized bed gasifier ?

We need to calculate the flow rate of air required for fluidization of the bed. We have a compressor which gives air at 7 bar and a 1 bar rotameter for measuring the flow rate in LPM. How can I...

16 December 2020 2,092 3 View

How to perform a mixed-effects Poisson regression with mutliple DVs?

I have a set of 6 related likert-type DVs, a within-subjects time variable (i.e. each DV was measured at 3 time points), and a categorical between-subjects IV (condition). Is there a way to...

16 December 2020 9,798 10 View