How can i use Balb/c mice (haplotype H2d) and C57BL/6 (haplotype H2b) to detect the inhibitory functions of some fusion proteins on mouse T cells? my system doesn't seem to be working. I isolated CD90.2 cells from spleen cells and and then use them as the responder cell while irradiated or mitomycin treated T depleted cells serves as the stimulator cell however I don't get a desirable response comparing the result to that of the control (syngeneic cells response) Anyone with an idea?