Anyone using REDExtract-N-Amp kit for small insects?

More Romana Iftikhar's questions See All

Can anyone pls provide me information regarding the libraries where from i can get the absorption data of different materials used in solar cells??

I want to create .abs file ( absorption) for simulating solar cell in SCAPS. Can anyone pls provide me information regarding the libraries (websites) where from i can get the absorption data of...

10 February 2021 6,034 7 View

Separation of protein via Akta system gave broad peak during elution. I wonder is it normal to obtain broad peak ?

Greetings.. I am using Akta Pure system for my protein purification. I have optimized buffer system for protein elution. However, this time I got broad peak while eluting sample. For...

24 December 2020 393 7 View

While running 15% SDS gel, I have noticed some blurred blue coloration above sample protein. What it could be?

While running 15% SDS gel, I have noticed some blurred blue coloration above sample protein. I have attached figure for the convenience. Suggestion and answers in this regard will be highly...

28 October 2020 2,869 1 View

Desired membrane protein band isn't aligned with marker in SDS-PAGE gel. What could be the possible reason?

I am having problems regarding to protein separation via SDS-PAGE. As a newbie in this field, I have tried to optimize the conditions for protein induction. My protein size is in 20-25KDa range...

03 September 2020 8,243 11 View

How to get an XRD peak for 2nd coat (MgF2), which is coated on Cadmium Telluride glass substrate?

We coated Magnisum Fluoride (1.5 Molar) solution and Spin coated on Cadmium Telluride (CdTe) glass plate. Anealed (heated in oven) at 70*Celcius for 2 hours and then sent for XRD. But we got only...

19 August 2020 1,678 3 View

PLA(Poilylactic acid) sheet suppliers in pakistan ?

Does anyone know a supplier company provide PLA (Polylactic-acid) plastic sheets in pakistan? There's several companies products Lactic acid-(food suppliers in liquid form, This is not what I...

16 March 2020 724 3 View

Fail Safe N, Orwin method?

I need to calculate Fail-Safe N of my meta-analysed studies. I am having difficulty in understanding Orwin's method. Can anyone please elaborate with the help of an example?

03 March 2020 7,403 3 View

Usefulness of CMA lite version?

Can I do moderator analysis on the lite version of Comprehensive meta-analysis software (CMA)? Or is it necessary to get the Pro version of it to perform moderator analysis? Quick responses would...

02 March 2020 328 2 View

How to collect email addresses for a survey research?

I am conducting an empirical research and wondering how to gather around 2000-3000 respondents email ids, considering the response rate of 10 - 15%. Does empirical researchers who are regularly...

24 July 2019 8,776 4 View

Looking for a scale to measure sustainable HRM?

Is there any developed and tested scale to measure Sustainable Human Resources Management ?? If some one have it please share. thanks

10 March 2019 2,735 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Muhammad Tayyib Naseem

I had used Zymo insect microprep kit

That was very good for extracting DNA from single aphid

Ali Athafah Tomah

Hello

I hope you have good day

This protocol is for isolating dna from small tissue of insect preferably legs, head and wings. We are using this protocol. here a link of the protocol (dx.doi.org/10.17504/protocols.io.bc47iyzn)