Muhammad Tayyib Naseem

6 Questions 11 Answers 0 Followers

Questions related from Muhammad Tayyib Naseem

Is there any example of insect which was previously reported as non-vector of virus and then able to transmit the same virus in later investigations ?

I am searching for any example insect species of the plant virus which was previously reported as non-vector the in later investigation it was found to be good vector of same virus, due to Genetic...

27 April 2016 9,365 3 View

Where can I have my Aphid samples identified?

I have almost 9-10 unidentified specimens that could be the new species from Pakistan. I have sequences their COI 5' gene and from GeneBank they are not showing ID similarities more than 90-95 to...

10 December 2015 7,821 7 View

Can we report virus on partial RdRP gene sequence?

I have a partial sequence of RdRp gene from Carrot Mottle Virus (CMoV, Umbravirus) and Carrot red leaf virus associated RNA virus (CtRLVaRNA, unassigned). They have 81% and 75% ID with database...

06 May 2014 7,742 3 View

Does the melt curve difference depend upon number of copied that are present in plasmid/cDNA or just on the product sequence difference?

I have used primer set F1R1 for qPCR with syber green, in that I used 1ul Plasmid as positive control of published sequence and my cDNA as target. The Ct value for Plasmids was 18 and for cDNA was...

26 April 2014 8,647 2 View

Is RdRp gene fragment enough to describe the CMD virus complex?

I have CtRLV 210bp fragment from RdRp gene that shows sequence ID with CYDV 91%, does it shows this could be different virus or strain. The other two members CMoV and CtRLVaRNA also have just 81%...

06 November 2013 899 8 View

What are the alternatives for Standard Curve in Quantitative RT-qPCR of RNA Virus instead of In Vitro Transcript?

I am trying to do the InVitro Transcript, but it's not working for me. I have only 2 months remaining for my research in the USA. Can I try something else like cDNA or total RNA to draw the Std...

04 February 2013 5,919 4 View