In California we have over 5,000 species of native plants, and no lands so far set aside to preserve those native plants in managed refugia, where the exotic plants are managed to help protect the native species for perpetuity. A Botanical Ark could be in situ living seed banks, that could preserve the plant species, along with their co-evolved insects and birds, etc. I am estimating that to preserve our 5,000 species we need at least 600 refugia of at least one hectare each, scattered across California about every 10 kilometers in each direction. The costs to establish them, needs to be the land costs, plus an annual management cost to keep exotic plants and exotic animals out, and to improve the sites by direct seeding of the local native seeds.