I´m looking for the best diluent for total antioxidant capacity in serum or plasma.
RNase-free water/ normal saline/ PBS/ 0.1M Tris-HCl (pH 7.4): in that order
Thank you very much Ebenezer. I used PBS but did not work. I will try your suggestions.
Dear Molina,
For me, I use 0.1 M potassium phosphate buffer (pH 7.4). I used to perform the activity assay at 25°C.
Hope for the best.
Thank you so much Debrapasad, we perform the activity assay with ABTS at 25 C too.
Hello, Raul
Which method you use to measure the total antioxidant capacity? Depending on the technique you use and how you want to preserve the samples, will be the most suitable diluent.
In total GSH assay, for example, we use phosphate buffer pH = 7 but we use it in the day and discard. In the case of wanting to preserve the dilution for future measurements, however, we use buffer Tris-base pH = 7.6.
I hope this is useful.
Regards,
Carolina
Thank you Carolina for your suggestions. We use ABTS to quantify total antioxidant capacity in blood serum samples. We are going to use tris- base. Thanks again!!!
Dear Raul Diaz Molina
For ABTS radical scavenging assay, 0.1 M potassium phosphate buffer (pH 7.4) is the preferred diluent followed by methanol.
Regards
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