70% well kill the cell. You must use less than 50 % to keep cell alive. Regards.

Dear Catarina,

in my experience with HaCaT keratinocytes, Ive always used final concentrations lower than 0.1% without citotoxicity issues.

Of course, you'll have to test it on your own cells, as the tolerated concentration may be different from one cell lines to another.

Remember to add a vehicle control, to exclude any biological effects from the vehicle itself.

Kind regards

Paolo

for HaCaT kerfatinocvytes: max 0.5% EtOH