EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

How can I determine which dye I'm dealing with?

I am currently analyzing the composition of an unknown solution. It is a blue dye solution, but there are an awful lot of blue dyes. I have already analyzed the UV-VIS spectrum, the wavelength is...

08 October 2020 4,651 4 View

Management Innovation and Bahavior economic?

Hello guys! I'm looking for studies that address behavioral economics in Management innovation. Especially regarding the decision-making process.

13 September 2020 8,687 3 View

How to overlay and align different scale raster?

I need to overlay two rasters and the pixels of they must be in the same position to each other. I need this to perform a ecological niche model using a package in R. For this end all images must...

20 August 2020 9,503 4 View

Does anyone know how to quantify C-S-H in cementitious materials using XRD?

10 August 2020 4,207 11 View

Can I performe behavioral tests in the same rats at PND30 and PND60?

Hi! Anyone knows whether I can performe behavioral tests in rats at PND30 and then, repeate them at PND60? Or this is not correct? Thanks.

20 July 2020 3,659 3 View

Load-Displacement curves with XFEM in Abaqus?

Hi everyone, I am working with abaqus by using XFEM. I am modelling a Single Lap Joint and I have simulated the crack propagation within the adhesive. Nonetheless, I also want to extract the...

18 March 2020 7,688 5 View

Where can I find public medical image datasets?

Histological, X-ray or Endoscopic images, for example.

13 February 2020 3,674 3 View

What is the best way to isolate viable TILs from mammary mouse tumor?

It's been a while I'm trying to isolate tumor-infiltrating lymphocytes from 4T1 mice tumor, but I haven't been successful so far. I already tried different tumor processing protocols, but all of...

21 October 2019 6,898 3 View

How we can solve the metals demand and its increasingly scarcity on earth within sustainable ways?

Hey every one! Indeed, we need to improve how we use our resources, mainly the metals, those are base materials for humans activities. Thus, this questions aimed to discuss about solutions to...

22 March 2019 9,032 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

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02 March 2021 3,060 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View