Paolo Governa

4 Questions 10 Answers 0 Followers

Questions related from Paolo Governa

What is the correct phenol red permeability through Caco2 monolayer?

Dear all, I am performing Caco2 permeability experiments using transwell inserts and I am using phenol red permeability assay to verify the monolayer integrity. What is the maximum phenol red...

07 March 2019 6,842 6 View

How can I induce CYP3A4 expression in Caco2 cells?

Dear all, I am using Caco2 cells to study intestinal permeability of natural products. Caco2 cells does not express CYP3A4, which is important for first pass metabolism and can hence influence...

21 March 2018 8,367 0 View

What is the best way to extract and concentrate proteins from large seawater volumes, under non-denaturing conditions?

Hi everybody, I am dealing with protein naturally suspended in seawater. I filter freshly-sampled seawater volumes (about 10 L usually) through GF/F filters and extract the trapped proteinaceous...

10 November 2016 8,012 4 View

How could I generate a pharmacophore representation of several docking results?

I docked many ligands into one protein and would like to make a pharmacophore representation of the better docking results. Is there any free software that allow me to use the docking results as...

26 August 2016 8,458 6 View