I need test methods to determine antinutritional factors (total alkaloids, saponin, tannin, etc.) in animal feed samples.
Tannic acid when reacts with Folin-ciocalteu reagent in alkaline pH gives absorbance max. at 725nm. Phenolic compounds also give the same reaction they can be estimated by the same mehod. Refer J. Sci. Food Agric. 1993. 16;161-165
Book:
Harinder P. S. Makkar, S. Siddhuraju, Klaus Becker. Plant Secondary Metabolites (Series: Methods in Molecular Biology - 393). Humana Press, 2007
Dear Ghadir
Tannin-binding assay is a very effective mean to quantify tannin in direct relationship to their biological activities: see my publication in J Agr. Chem. Food sci for the method and my review in Animal Feed Sci Technol. These publication can be downloaded from this site, or from my publication site (see link in the data set).
Nissim
Contact Dr. Marcos Soto Hernández, Professor at Colegio de Postgraduados, Campus Montecillo. Dr. Soto is an expert identifying secundary compounds in plat tissues, he recently performed some analyses for our research team.
e-mail: [email protected]
Please, check out the contributions of Carmen Cuadrado or Carmen Burbano in ResearchGate. You'll find many references of the methods.
H.P.S. Makkar has a protocol for tannins in a publication. Google search to find the publication
I think this is a protocol that you are looking for about Tannin.
According to my investigation:
Chemical analyses
alkaloid content was determined according to Wiewiórowski and Skolik (1959)
Wiewiórowski M., Skolik J., 1959. Photometrische Mikrobestimmung der Lupin- Alkaloids. Ann.Chem. 33, 461-46
In: Niwińska B. (2001). The nutritive value of Poland-grown lupin cultivar seeds for ruminants. J. Anim and Feed Sci., 10, 91-101.
The butanol-HCl method by Terril et al is a reliable method for condensed tannin determinations, we use predominantly for forage samples but have also used for grains. NB: The standard curve needs to be extracted from a similar sample type. Terrill, T.H., Rowan, A.M., Douglas, G.B. and Barry, T.N. (1992). Determination of Extractable and Bound Condensed Tannin Concentrations in Forage Plants, Protein Concentrate Meals and Cereal Grains. J.Sci Food Agric; 58, 321-329.
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