Anyone know of any FRET-capable Fluorescent Protein pairs where the donor excitation wavelength is >500 nm?

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David Oppenheimer

What is the emission wavelength of the protein that is excited by 500nm light? That will determine which FRET acceptor you could use.

Here is a link to an interactive fluorescent protein ex/em chart. It should be helpful in narrowing down your search.

http://nic.ucsf.edu/FPvisualization/