I am trying to stain spinal cord after cryo-sectioning.
I got this protocol in IHCWORLD.COM,
please try once.
Bancroft's histological technique book shows the following protocol as in attached file page 359-360.
Mahendra Pratap Singh Tomar thank-you very much sir.
I got this protocol in library.med.Utah.edu
I am not understanding how each author is deriving the equations and the working principle. Individual authors are giving their own working principle explanation for different circuit topology....
12 February 2021 496 1 View
I want to plot the monthly average electric production data and cash flow data in another software to get a more clear graph. For this, I need to extract the monthly or yearly data and not the...
11 February 2021 2,740 2 View
Do diaphragmatic breathing and speech therapy actually help? How often did you require multidisciplinary team approach?
17 January 2021 5,998 0 View
provided that all the criteria for diagnosis has been fulfilled and alternative diagnosis are also ruled out. The patient has coagulopathy and transjugular biopsy wont be possible due to resource...
13 January 2021 8,935 4 View
I wish to examine the impact of environmental degradation on human security in Bangladesh.
11 January 2021 3,282 6 View
I want to conduct a research which is based on time series data ( Suppose, Relation between GDP, Foreign direct investment, Import, Export). Previously I worked with panel dataset and I know the...
31 December 2020 1,207 6 View
Dear Friends: 64 bit Gaussian 09 software for windows is more expensive than 32-bit Gaussian 09. In our work, we deal with coordination compounds with about 50 atoms. So, can we use 32-bit...
27 December 2020 2,877 2 View
Can anybody give me the following writer book? Helen Deresky "International Management"
26 December 2020 5,109 5 View
Can someone help me to realize the basic concept of user power allocation for a wireless heterogenous OFDM network where there is a macro cell and inside it some femto cells. What is the basic...
01 December 2020 4,039 1 View
Now a days many researchers are depositing their research paper draft as preprint in various web-based option, those are not peer reviewed yet or may submitted already or will be submitted in a...
29 November 2020 6,849 2 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...
24 February 2021 4,618 4 View
24 February 2021 3,348 2 View
Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...
24 February 2021 9,578 4 View
Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...
24 February 2021 5,430 3 View
Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...
24 February 2021 9,902 5 View