I have difficulties in growing HD11 cells. "In house" they have been cultivated in RPMI + 10% FBS, but I can see that others have used RPMI +2.5% chicken serum + 7.5% FBS or DMEM + 5 or 10% chicken serum.
When subcultivating them, how confluent should they be? And should I use Trypsin or a cell scraper for deattachement?
Any other precautions, tips or tricks? :)
Dear Gry. Do you still have problems? If possible I would consider 41 degrees depending on your purpose!! Where did you get the cells from? More than 1 type HD11s exist!
Dear Gry, I would like to purchase chicken macrophage cell line. Where did You buy your HD11 cell line ???? best, Maria
Dear Gry, some people in our lab in the past have grown those cells. Went to the pubmed to pull up the paper from JBC 2005 280:20604. In the methods section: "HD-11 cells were generously provided by Dr. T. Graf (EMBO, Heidleberg, Germany) and grown in monolayers in Dulbecco's modified Eagle's medium (DMEM; Invitrogen) supplemented with 15% fetal bovine serum (FBS; Omega, Tarzana, CA), 4 mM L-glutamine, 100 units/ml penicillin, and 100 μg/ml streptomycin (Invitrogen) at 37 °C in a humidified atmosphere of 5% CO2 and 95% air." My impression was that the cells grew rather vigorously. hope that helps.
Dear all,
Can anyone of you help me with some HD11 cells? I wanted to buy but I can't find them on the ATCC collection. You all got it from Dr T Graf? Thanks
Dear all,
Can any one help me with HD11 cells?? I am in University of Adelaide and want to use HD11 Cell line for research??
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