Anyone have experience working with chicken macrophage cell line HD-11? Which media to use? How to subcultivate them?

I have difficulties in growing HD11 cells. "In house" they have been cultivated in RPMI + 10% FBS, but I can see that others have used RPMI +2.5% chicken serum + 7.5% FBS or DMEM + 5 or 10%...

08 August 2013 2,462 5 View