Anyone have experience with Borax Agarose gel electrophoresis for RNA ?

08 August 2013 1 3K Report

I use them for DNA gels regularly. Any precautions for RNA?

http://www.mudphudder.com/wp-content/uploads/2008/11/jr-brody-and-se-kern-biotechniques-2004.pdf

Hi Norbert,

I guess you might have found the answer to your question by now, but if not, here is a link to another publication from the same authors, for running Tris and MOPS free RNA gels in Borax based buffers, and faster!

Best,

~a.

http://www.biotechniques.com/BiotechniquesJournal/2004/October/Ultra-fast-high-resolution-agarose-electrophoresis-of-DNA-and-RNA-using-low-molarity-conductive-media/biotechniques-117338.html

Badges
Science topic

More Norbert Bittner's questions See All

Does anyone have experience with Glycosilation modification with PES ultrafiltration tubes?

Does anyone knows if PES (Polyethersulfone) in ultrafiltration tubes is modifying or changing the glucosilation (patterns) of proteins ?

07 August 2014 3,974 4 View

Is the Commasie used in BN-PAGE interfering with protein activity after gel elution of cut out bands?

Do I have to destain before eluting out my protein of interest to keep it active?

08 September 2013 2,506 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View