I have a problem with some sequencing results (Sanger seq., germinal DNA changes) in one exon.

I´ve obtained different results (SNP - normal allele/ heterozygote) depending on the sense of the primer. I repeated it 3-times (including new amplification) and it´s still the same. This potential change is known as nonpatogenic polymorphism, but not in Caucasians as far as I know.

Thanks.