What are the filters that use for Lateral Flow Immunoassay system?

Lateral Flow Immunoassay system were used for newborn screening test and these systems consist of some parts including plasma separation membrane part, I found lots of plasma separation membrane...

09 October 2019 6,408 1 View

What are post-operation managements in mice orthopedic surgery?

I did orthopedic surgery on my mouse yesterday and for surgical prophylaxis, I had 10 mg of intraperitoneal cefazolin injection 30min before surgery, then once every day for 5 days 10mg of...

08 September 2019 9,895 3 View

Can I use Nanodrop for checking DNA contamination/concentration in my RNA samples?

I used nano drop for checking RNA concentration of my samples and its was about 2000 ng/microliter and A260/280 was approximately 2 after that I asked myself what will happen if I tell nano drop...

08 September 2018 7,795 6 View

Is EDTA good for DNase I inactivation?

I used EDTA for DNase I inactivation in my samples, but I read that EDTA is Mg Chelator and cause some problem on quality of q-PCR results, and more to that I understand that it has also...

08 September 2018 5,179 4 View

How to treat lab utensils with DEPC water?

What's the DEPC water protocol for removing contamination from racks and other utensils in lab?

07 August 2018 9,108 7 View

What will happen if I use extra amount of RNA for cDNA synthesis?

07 August 2018 8,777 3 View

Can I use DNAase on old extracted RNA?

I want to know whether I can use DNAase on RNA which was frozen 2 years ago or not?

06 July 2018 7,174 3 View

What's the best cytoscape plug-in for drawing heat map?

I want to know the best cytoscape plug-in for drawing heat map, and if anybody know any other good way I'll be thankful to help me with that. Thank you

04 May 2018 8,146 2 View

Are the samples in series matrix files of microarray datasets in GEO comparable with each other?

I want to draw a trend of the expression of genes which i have and for doing so i want to know whether the samples in GEO series matrix (I mean GSMxxxxxx) are comparable with each other (sth like...

02 March 2018 4,767 3 View

Database for neuroscience

Can you help me with some database for neuroscience, for example fMRI database, or database which show underlying mechanisms of the brain, show the connection between brain and behavior,...

01 January 1970 8,891 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View