What are the filters that use for Lateral Flow Immunoassay system?

Lateral Flow Immunoassay system were used for newborn screening test and these systems consist of some parts including plasma separation membrane part, I found lots of plasma separation membrane...

09 October 2019 6,408 1 View

What are post-operation managements in mice orthopedic surgery?

I did orthopedic surgery on my mouse yesterday and for surgical prophylaxis, I had 10 mg of intraperitoneal cefazolin injection 30min before surgery, then once every day for 5 days 10mg of...

08 September 2019 9,895 3 View

Can I use Nanodrop for checking DNA contamination/concentration in my RNA samples?

I used nano drop for checking RNA concentration of my samples and its was about 2000 ng/microliter and A260/280 was approximately 2 after that I asked myself what will happen if I tell nano drop...

08 September 2018 7,795 6 View

Is EDTA good for DNase I inactivation?

I used EDTA for DNase I inactivation in my samples, but I read that EDTA is Mg Chelator and cause some problem on quality of q-PCR results, and more to that I understand that it has also...

08 September 2018 5,179 4 View

How to treat lab utensils with DEPC water?

What's the DEPC water protocol for removing contamination from racks and other utensils in lab?

07 August 2018 9,108 7 View

What will happen if I use extra amount of RNA for cDNA synthesis?

07 August 2018 8,777 3 View

What's the best cytoscape plug-in for drawing heat map?

I want to know the best cytoscape plug-in for drawing heat map, and if anybody know any other good way I'll be thankful to help me with that. Thank you

04 May 2018 8,146 2 View

Are the samples in series matrix files of microarray datasets in GEO comparable with each other?

I want to draw a trend of the expression of genes which i have and for doing so i want to know whether the samples in GEO series matrix (I mean GSMxxxxxx) are comparable with each other (sth like...

02 March 2018 4,767 3 View

Anyone can help me to find novel node in my network base on graph theory? I want to find novel genes but i don't know how to select them?

I analyze my network with cytoscape and now i have lots of their trait like betweenness, closeness,degree and... but i don't know how to choose my novel node base on these things.

31 December 2017 1,262 1 View

Database for neuroscience

Can you help me with some database for neuroscience, for example fMRI database, or database which show underlying mechanisms of the brain, show the connection between brain and behavior,...

01 January 1970 8,891 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View