Anyone already worked with sigma Anti-HA antibody for IHC, please share your experience?

13 June 2020 0 5K Report

I have two choices for IHC either to go with Mouse Monoclonal Anti-HA (Clone HA-7) H9658 or rabbit poly clonal affinity isolated antibody both are from sigma.

1) I know in terms of specificity monoclonal would work better, please share your experience or suggestions too.

2) Secondly in light of your experience could you please recommend any good working dilution for IHC? I am unable to find any dilution range from sds or publications. Its some kind of urgent experiment, antibody titration or using various concentration and then selecting one wont help this moment.

Note: I have to do IHC on mouse tissues.

I would be grateful if anyone having similar experience could suggest about this situation.

Thank you in anticipation.

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