Rizwana Ali

8 Questions 6 Answers 0 Followers

Questions related from Rizwana Ali

How long it takes newly regenerating skeletal muscle fibers to be mature after injury?

Usually skeletal muscle after injury undergo regeneration process following by all key phases of degeneration, inflammation and so on so until regeneration of fibers. For example we usually...

14 April 2021 2,092 2 View

Anyone already worked with sigma Anti-HA antibody for IHC, please share your experience?

I have two choices for IHC either to go with Mouse Monoclonal Anti-HA (Clone HA-7) H9658 or rabbit poly clonal affinity isolated antibody both are from sigma. 1) I know in terms of specificity...

13 June 2020 4,926 0 View

Does anyone has experienced working with NIK SM1 (NIK;NF-κB inducing kinase inhibitor)??

Which working concentration is recommended to get perfect inhibition of NIK (NF-κB inducing kinase) by NIK SM1? It would be great if I can get help from your valuable experience. Thank you

23 July 2019 9,138 3 View

Can anyone please suggest me how to over-come C2C12 de-attachment issue?

I changed every thing media etc like new freshly prepared, deattachment of C2C12 myofibers persist. One of my old plate I forgot to discard after few days I saw strange movement there, what It can...

09 April 2019 785 0 View

Can anyone please help me to solve the issue with differentiation of C2C12?

1) I really stuck quite suddenly with my experiments because of strange issue with C2C12, upon differentiation the C2C12 (myofibers) layer become shrink-ed to one point and totally de-attach from...

20 March 2019 2,524 0 View

I am looking for few valid/working detailed protocols for mouse behavioral tests?

Anyone working on behavioral neurology or using mouse models for behavioral testing, could you please share the valid detailed protocols regarding below mentioned tests; 1) Grip Strength 2)Using...

11 February 2019 4,443 5 View

Can anyone please suggest me best working inhibitor for NIK (NF-κB inducing kinase)?

Does any one has information OR used NIK inhibitor that is commercially available? As well as TRAF activators too? If some one already used please recommend me too as well their working...

23 January 2019 5,716 2 View

Can anyone please provide the detailed protocol for skeletal muscles isolation for protein extraction as well as BCA assay?

As a beginner I need detailed protocol for protein isolation from specific skeletal muscles of mice such as TA, GAS etc, (for western blot analysis) especially BCA assay I never done before, how...

27 November 2018 5,362 3 View