ATR-FTIR can be used to probe the chemistry of colloidal samples without drying or using a flow cell, allowing in situ measurements of molecular interactions and compositions. Strategies to account for the broad water peak include spectral subtraction, deconvolution techniques, and using alternative ATR crystals. Studies have demonstrated the versatility of ATR-FTIR in analyzing colloidal systems and mitigating water peak interference.

Thank you for your response. I'm not sure that subtraction of water peak will work in my case as it might produce false spectral features. But I can try other deconvolution techniques as you mentioned.

If spectral subtraction may introduce artifacts in your ATR-FTIR analysis, consider using advanced deconvolution techniques to resolve overlapping peaks without removing the water peak. Additionally, employing baseline correction, temperature control, or alternative solvents can further enhance the clarity of your spectra.