I precipitated saponins from butanol fraction using acetone. Saponins are mostly steroidal and highly polar . i tried many mobile phase systems on silica TLC but could not get a good separation.
NB. i have no RP plates. I'd be grateful for any suggestions
Dear Sir. Concerning your issue about the solvent systems on TLC for separation of saponins . TLC is becoming a rather supporting technique for the analysis of saponins fractions from CC. It has been used for confirmation of purity and identity of isolated compounds. HPLC is one of the most versatile of the separation techniques. However, the absence of a chromophore in most saponins hampers their detection under ultraviolet light and only allows nonspecific detection at 200–210 nm. The problem is that at this wavelength, components other than saponins may also absorb in this region thus making saponin determination difficult. This problem has recently been overcome by evaporative light scattering detection (ELSD) which allows the detection that measures the scattered light generated by the nonvolatile particles of analytes produced by nebulization into droplets of the effluent. ELSD is a universal, nonspecific detection method that can provide a stable baseline even with gradient elution. I think the following below attached files may help you in your analysis:
Thanks
dear Professor Islam
thank you so much for your reply
the problem is that i couldn't find any cc device in egypt and my saponins appear to be very very close
even when i performed lc/ms/ms it showed compounds having almost the same fragmentation pattern so i need to separate them first and then identify them.
I usually take a mixture of petroleum ether and diethyl ether for my steroidal compounds in TLC. May be it can work with yours also.
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