What are examples of data-intensive research leading to totally new insights?

Data-intensive research is still all the rage these days. Breakthroughs in AI (and especially NLP) seem likely to only accelerate its adoption. But can anybody provide examples (preferably from...

17 January 2021 10,008 4 View

Can somebody recommend a dual expression vector?

09 January 2021 2,679 2 View

How to merge two Bold runs (rsfmri)?

Dear all, I have 2 Bold runs of a resting state MRI of the same subject. I want to combine these two into a single bold run. I habe tried so far to use fslmerge by time, but it seems not to be...

23 October 2020 4,480 1 View

How to estimate sample size for mixed ANOVA with several factors?

I would like to estimate the sample size for a 3b x 2b x 2w x 3w x 6w ANOVA [b=between subject factor; w=within subject factor]. I do not know much about the estimated effect size or...

16 July 2020 9,457 3 View

Where can I find information to justify the crosslinking of an antibody?

Hi all, I recently did a project where I did carboxyl-to-amine immobilisation with a monoclonal interferon gamma antibody (human ELISA development kit, Mabtech). I had carboxyl groups on a carbon...

30 May 2019 6,335 7 View

Are there any studies on the effects of the duration of short rests (breaks) in work on VDU?

need some valid results on the effects of short breaks while working with the computer and display.

20 June 2018 809 4 View

Is there a Software to depict multi-channel linguistic data transcripts?

Related to my PhD-research I am looking for software that is capable of displaying multi-channel transcripts of linguistic data. More precisely, I do have video data, that includes writte (chat)...

25 February 2018 5,263 2 View

Reverse transcriptase with 5'3' exo activity

Hi! I am looking for a reverse transcriptase that has no strand displacement but rather 5' to 3' exonuclease activity. The closest thing I found is Bst polymerase from NEB but that is only a weak...

24 July 2017 6,312 2 View

Is it allowed to conduct an ANOVA with a within-factor that has no variability?

Dear folks, does anyone know whether it is allowed to calculate a repeated measures ANOVA where one factor has no variability? E.g. I have 3 types of errors (factor categories: A,B,C) and 6 action...

24 July 2017 662 3 View

How to deal with strange AIC and BIC values in a latent class analysis?

19 June 2017 9,674 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View