Dear all,

By means of using divergent primers, I have been trying to amplify lariat introns from mouse bone marrow/ primary neuronal cultures and 3T3 cells to study circular RNAs. However, I get amplification only when circular RNAs are formed by exons and not when they are formed by introns.

Any suggestions/tips by people experienced in this kind of study are welcome.

Thanks in advance,

Utkarsh