Hi Allen, please see this procedure:

Bressan EA, Rossi ML, Gerald LT, Figueira A. Extraction of high-quality DNA

from ethanol-preserved tropical plant tissues. BMC Res Notes. 2014 Apr 24;7:268.

doi: 10.1186/1756-0500-7-268. PubMed PMID: 24761774; PubMed Central PMCID:

PMC4005624.

DNA extraction

For DNA extraction, 50-mg leaf samples from both conservation treatments (ethanol or frozen in liquid nitrogen) were finely ground in liquid nitrogen. The pulverized samples were incubated in buffer (2% cetyltrimethylammonium bromide, 1.4 M NaCl, 100 mM Tris–HCl [pH 8.0], 20 mM EDTA [pH 8.0], 1% polyvinylpyrrolidone [mass weight 10,000], 0.2% β-mercaptoethanol, and 0.1 mg mL-1 proteinase K) at 55°C for 60 min [9]. After this step, the solution was extracted twice with chloroform:isoamyl-alcohol (24:1 v/v). DNA was precipitated by the addition of cold isopropanol to the solution followed by centrifugation; the resulting pellet was washed with 70% ethanol and allowed to air dry. The DNA pellet was resuspended in 50 μL TE buffer (10 mM Tris–HCl [pH 8.0] and 0.1 mM EDTA [pH 8.0]) containing ribonuclease A (10 μg mL-1) and incubated at 37°C for 30 min [10].

DNA concentration and quality

DNA concentration was estimated using a DyNA Quant 2000 fluorometer (Amersham Biociences, Buckinghamshire, UK) and a NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). DNA quality was checked by electrophoresis of 50-ng aliquots on a 0.8% agarose gel stained with SYBR Gold (Invitrogen, Eugene, OR, USA).

If you the supernatant of swab sample for extract DNA, it may be not good to get DNA.

Please vortex well the tube and centrifuge down the pellet. Using the pellet for extract DNA. It may work.

Hi, use the kit for DNA extraction from Macherey-Nagel (http://www.mn-net.com/tabid/11422/default.aspx). In the kit for DNA extraction from tissue you have good protocol for extraction and it is working very well!Good luck!

Hi Jenn,

we have protocols for this on our website www.starworms.org

Piet