Origin - Multiple peak fitting - File limits in batch processing mode?

Dear all, I am having some issues with the multiple peak fitting function in Origin (2018). My workflow is as follows: My data are baseline corrected and normalized between 0 and 1 after...

24 February 2020 8,479 2 View

Micro - small scale desalination technologies for single homes and small communities?

22 August 2016 2,324 4 View

Does anyone know of any Chinese, Indian or Russian mathematicians with an interest in fractals and the M set?

We are looking for Chinese, Indian and Russian mathematicians with a passion for fractals and the M set, who would be interested in being interviewed for the film we are making called 'Beyond the...

19 March 2015 6,610 12 View

Can anyone tell me who was the first to propose that the metabolic transformation of cancer cells confers a survival advantage?

The metabolic transformation of cancer cells as described by Warburg now appears to be a considered a hallmark of cancer. The literature appears to consider that this metabolic transformation...

06 March 2015 439 6 View

Has anyone successfully expressed with pCold-I?

I am a first time user and I obtained expression both in Induced and uninduced (even more expression) using BL21 (DE3) after culturing in LB medium and inducing with 1 mM IPTG. It looks very...

09 November 2014 6,191 3 View

Indirect ELISA using mouse antisera

I have been trying to develop an ELISA assay to identify the presence of the whole parasite in culture by targeting one of its membrane protein. I recently found out that there is no signal...

02 April 2013 9,275 6 View

Does anyone know of any human physiology modelling software that we could "integrate" with the virtual patient simulations we are creating?

The software would for example model the effects of changes in blood pressure, heart rate and rhythm, skin colour change, in response to various interventions such a drug administration, change in...

17 January 2013 1,184 8 View

Do you intend to bring the Barthes project up to date, i.e. Modern Mythologies ?

We here at Loughborough University in the UK have a Research Group to investigate "The Arts in the Public Sphere", and we are holding a conference at our London campus on "Modern Mythologies"...

01 January 1970 2,819 1 View

What's New in Teaching Public Health?

Many talking points that seemed fringe or radical 12-months ago are no longer so. The mainstream media are taking a closer look at reparations, at economic inequality, and at restorative justice....

01 January 1970 5,964 28 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View