I'm looking for a good but affordable chemiluminescence imaging system to scan western blots - probably using ECL. I just starting searching, but it seems the "Li-COR C-DiGit blot scanner" is popular. Any experience with that or others, regarding easy of use and sensitivity is welcome. Are there any others I should look into?
We have GE ImageQuant 500. It's really great.
http://www.gelifesciences.com/webapp/wcs/stores/servlet/productById/en/GELifeSciences-uk/29005063
We have a Biorad ChemiDoc that works nicely as well and also has the capability to image multiplex fluorescent western blotting.
http://www.bio-rad.com/en-us/product/chemidoc-imaging-systems/chemidoc-mp-system
Look into ImageQuant from GE. You can do all sort of things like imaging DNA gels and if you find the filters you can also image fluorescence (WB probed with a fluorescent secondary). We also have the Licor in the lab and is an extremely reliable imaging system. Very good but it requires that you buy secondary antibodies that are fluorescent which can end up being expensive
thank you! Looking at the prices, I don't think we are quite ready for an ImageQuant or ChemiDoc, since we aren't doing any DNA gels or multiplex yet.
I'm glad the Licor performs well and scheduled a demo for next week to try with their ECL and one I have already, since theirs seems more expensive than most.
The C-DiGit Scanner is a good and very inexpensive option ($4995). Here's a link to some reviews on SelectScience:
http://www.selectscience.net/products/c-digit-blot-scanner-for-chemiluminescent-western-blots/?prodID=171659
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