Hi, I am trying to knock down a gene of mosquito using double stranded RNA. I made it using Ambion Megascript RNAi kit. In agarose gel, it looks good although the dsRNA size is shorter than the actual size (actual size is ~600bp, I got a good band in ~500bp). My problem is, I can not successfully knock down that gene with this dsRNA. I use dsGFP and uninjected mosquitoes as negative control. Anybody have any suggestions for me?