I tried to build this model by percutaneous injection to the right lung under the X-ray. But cancer cells are spread to the pleural cavity and left lung. Has anyone has any good idea？
It is challenging to create an orthotopic lung cancer model as it often involves a survival surgery. If you like, you could inject cancer cells i.v. that form tumor nodules in lungs. Although It is typically used as metastasis model, but it could also be seen as orthotopic lung cancer model.
Matrigel does help to hold tumor cells together. How many cells do you inject? I guess an ultrasound-guided injection may be more convenient for the operators than X-ray C-arm if it is available.
The other alternative is to inject tumor spheres derived from in vitro anchorage-free culture.
Thanks for your guys helping. Actually i already tried the Matrix gel, but it does not help. And because it's a lung, we can't use the ultrasound device.
Have you tried ultrasound on mouse lung? What did it look like? I never paid attention to mouse lung when I looked for mouse heart. But I assume those black area around the heart is mainly lung parenchyma and pleural space. I indeed used ultrasound guide for some lung biopsy in patients. I wonder it could be feasible for mouse (injection) too. Nonetheless, ultrasound might help to avoid injection into vessels or other structure, but may or may not help to ensure injection into lung parenchyma.
Regarding the issues of spreading into pleural cavity and the opposite side, several factors might be considered:
1.your cells may be very invasive or metastatic
2. cell leakage into pleural space related to injection procedure. did you induce pneumothorax during the procedure? how did you confirm the cells were injected into lung parenchyma?
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