Hello everybody,
There is a problem with Ziziphus jujuba micropropagation through the single node and shoot tip explant culture. The explants suffer from leave shedding, shoot tip necrosis, and dieback within two weeks of culturing on multiplication media. Until now, different multiplication media, including interactions of different nutrient media (MS, WPM, WPM, and QL) with different PGRs (BAP, Kin, TDZ, NAA, IBA in different concentrations) along with various complementary materials (Fe-EDDHA, glutamine, Ca-gluconate) were tested and, unfortunately, they have not been effective. In addition, we tested the media with manipulated CaCl2, Ca (NO3)2, and H3Bo3 (up to doubled concentrations). However, none of the treatments have been effective so far.
You can find some photos of the explants in the attachment.
I would be grateful if you share your experiences in this subject.
Best regards
Hello!
This is interesting question.
Did you try to shift pH of the media?
Did you try to use 1/2 Basal salts?
Also, you can join this group on PTC in WhatsApp and ask your question There. There are a lot of experienced colleagues in PTC:
https://chat.whatsapp.com/JxyKhcboNFj1hJd1NgwIKe
You can try liquid medium for shoot multiplication it might work.
There is one very good review which discuss all the aspects with many other plants examples.
https://link.springer.com/article/10.1007%2Fs00425-020-03449-4
It might be useful to you.
All the best.
Dear Lidia Samarina and Ashutosh Pathak,
Thank you for your helpful comments and suggestions. I've also tried the liquid medium, but the necrosis and dieback intensity increased.
And also, I've tested the media with 1/2 macroelements concentration, but it didn't work. The pH of the media tested from 5.8 to 6.3, and there was not observed a significant difference.
Best regards
Ruhollah Abdolinejad
We also observed similar problems in certain Lamiaceae members and got rid of the problem using meta Topolin and doubling the calcium and boron concentrations. I would suggest you use meta Topolin and a slight increase in calcium and boron concentration in the medium can reduce the shoot tip necrosis.
Please go through the following article for reference,
Article Shoot tip necrosis of in vitro plant cultures: a reappraisal...
Dear M. Manokari,
Thank you for your helpful comment, I will try Meta-topolin.
Hi.
Worked on Jujube ten years ago.
Did not use nodes. Used young leaves.
Got shoots on:
WPM plus 1 micro M TDZ plus 1 micro M IAA,
PH 5.7
I have the same thought as Dr. Samarina. After autoclaving, pH of the culture medium might be lower than 5; which can interfere the absorption of some minerals, such as calcium, causing the the shoot tip necrosis. Therefore, besides the slight increase in Calcium concentration as suggested by Dr. Manokari, how about the slight shift of pH to alkaline side as Jujube can grow on soil very alkaline.
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