IS there a specific procedure to thaw cells from liquid nitrogen?

Hi all! I have been thawing a few cell line stocks we had kept in liquid nitrogen and faced a couple of problems not being able to get the cells adhere to the T25 flask easily. I am not sure if...

27 December 2020 5,525 2 View

How can we split cells from T25 to T75 flasks?

Hi all, I am trying to wrap my head around splitting cells into different surface-area flasks. I could really use your help! I currently have 8 T-25 flasks of HEK-293T cells. I am familiar with...

04 November 2020 3,023 4 View

Can I use low concentrate Sulfuric acid into drip irrigation?

Generally water alkalinity to clot the dripper nozzle due to salt formation. So bring down the irrigation water pH to 5.5 to 6.5 may useful for prevent for salt formation as well as crop...

31 August 2020 675 3 View

How do I solve this error when trying to execute the GROMACS command "xpm2ps"?

Hi all, I hope you could help me out with this question in GROMACS. I am trying to use the "xpm2ps" command in GROMACS to output a postscript file from an .xpm file. However, I keep on getting...

15 April 2020 6,028 2 View

What is the best method for measuring copper accumulation in bacteria and fungus?

I am doing research on microbial remediation. How to measure the copper accumulation in bacteria and fungus ? The supernatant can be measured by AAS or ICP-OES OR Acid digestion of bacterial...

16 December 2017 4,447 3 View

Does further heating process make any structural variation in metal ferrite sample ?

05 October 2017 5,675 3 View

How do we calculate wt% for composites ?

i want to make composite with two different oxide materials in review papers one material weight is fixed in gram another material was added to different wt% so i don't know how to calculate the...

04 October 2017 3,405 5 View

Hello, how do I interpret the below confocal images?

I recently did IHC staining of mouse brain tissues with four different primary antibodies, but am unsure as to how I am supposed to assess and troubleshoot these confocal images. The following...

28 September 2017 9,502 3 View

What is the best method to concentrate a large volume of protein samples?

05 August 2014 2,219 5 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View