Can anybody suggest a spectrophotometric method for detecting the presence of selenocysteine in a protein sample?
A number of methods are available, if u dig n try to search, i just found one among many many with relevance, bring ur mind in constructive science than else's, u will do much better... find attached paper below.. hope this helps..
Lots of protein remains bound to the matrix even after elution with buffer containing 500mM Imidazole.
05 June 2016 1,251 3 View
I have a His-tagged protein of ~70kDa, cloned into pET23(a) expression vector and the protein expresses in BL21 (DE3) cells when induced with 1mM IPTG and grown at 37C for 4hr after induction. The...
02 March 2016 256 9 View
I am using pET23(a) vector having a C-terminal His-tag to express a gene of 1.7kb. But as the reverse primer sequence includes stop codon , I think the his-tag expression is hindered. And...
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How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
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Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
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Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
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Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View