Can anybody suggest a spectrophotometric method for detecting the presence of selenocysteine in a protein sample?
A number of methods are available, if u dig n try to search, i just found one among many many with relevance, bring ur mind in constructive science than else's, u will do much better... find attached paper below.. hope this helps..
Lots of protein remains bound to the matrix even after elution with buffer containing 500mM Imidazole.
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kindly reply me. Thanking you in advance.
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