My synthetic gene needs not only codon optimization but also avoiding potential intron splicing for human host expression. Does any one know this kind of software?
Do I need to worry about the hidden intron when design a synthetic long gene?
Is splicing really highly conserved in Eukaryotic hosts? Can I take a piece of other species, say Drosophila, genomic DNA coding region rather than cDNA, into human cell under proper promoter and get transcribed and spliced as in drosophila?
One reason that you use cDNA for cross-species gene expression is because different organisms do have slight differences in exon splicing. Another is differences in gene size. Human genes are massive compared to other animals.
Note: "splice" means "to join". You splice exons together.
Codon optimization is a separate consideration. I'd recommend you talk with your advisor about your project.
And take a look at the existing literature: Article Parallel evolution of a splicing program controlling neurona...
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