I am trying to see the differences in protein body surface and foldings among normal and high digestible protein varieties. Can any one share me a protocol on sample preparation and imaging using CT or electron microscopy. Your cooperation is highly appreciated.q
Dear Tadessee Fikre Teferra,
In relation to imaging Protein bodies and starches from sorghum endosperm while reducing the time and effort of the CT and electron microscopy, I would suggest imbibing your sorghum seeds in water then use a froze microtome to cut longitidudinal cross sections.
Harvest the cross sections and use protein or starch dyes to be indicators of the relative presence and concentration of starch and protein accumulations.
I would expect most of your starch in the endosperm and most of the protein in the embryo and seed coat layer of the aleurone. Tradition Iodine stain has been a key starch indicator and I think Lowry stain was one of protein indicators you may want to search the literature for stains and try several in your studies. I believe this will save you a lot of time and you might complement the studies you are already doing.
Take a lot of pictures and share them.
Hope this information was helpful. PRH
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