How yo prepare samples for RNA extraction?

I really need help. I tried finding a protocol on how to prepare samples for gene expression . I am working on Antimicrobial activity of a medicinal plant and I would like to check its effect on...

30 December 2020 7,112 2 View

Plant extracts on expression of virulence related genes?

Good day. I would like to extract total RNA using a kit from MRSA and candida for gene expression. i tried sub-culturing standardized inoculum (50ul) with 500ul of my plant extract sub-MIC and...

23 December 2020 5,076 3 View

Silver nanoparticles synthesis using plant extract?

Please i still want more clarity on synthesis of silver nanoparticles. I tried different parameters like changing concentration of plant extract, for silver nitrate and so on. I observed color...

09 December 2020 1,546 3 View

Silver nanoparticles SPR peak and SEM image?

I tried synthesizing silver nanoparticles using plant extract. 1mM has not changed any colour. i then tried using 10mM and colour changed from green brown. i was monitoring the biosynthesis using...

07 December 2020 3,547 3 View

Western blot (beginner) protein loading quantity?

Hi everyone, I am an extreme amateur when it comes to western blots. I am taking over someone else's experiment (he left the position). He left his samples in the freezer and gave me the sheet...

03 November 2020 2,534 2 View

What is a good "analyte-free" method blank to use in cannabis testing?

I am looking for a method blank which I can use in the heavy metals, terpene, solvents, cannabinoid, pesticide, and microbial analyses I do in my analytical lab on cannabis samples. I have tried...

18 August 2020 320 1 View

WST-1 assay inaccuracy?

We are using the WST-1 assay to look at cell viability; however, when we add the dye to one of our cell lines, we are not getting accurate results. Under the microscope we see an increase of cell...

01 May 2018 4,449 3 View

How to compensate for unequal sample sizes in multivariate tests?

12 December 2017 308 3 View

Does anyone know how FBS promotes cell adhesion?

Studying cell migration of glioblastoma primary cell lines and we're looking for alternatives to FBS to promote adhesion without altering the migration phenotype. Does anyone know the particular...

25 May 2017 6,246 2 View

Would a phenomenological or grounded theory design be appropriate for my research question?

I am writing a qualitative research proposal. The purpose of my research is to explore nurses perceptions of practices (both personal and systemic) in emergency medicine that may be ageist in...

12 November 2016 1,671 28 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View