Good day.
I did qPCR to compare expression level of genes between treated and non treated. i was going through some papers and reported fold change +-SD. i am calculating my fold change using delta delta-ct method. Now my question is that which standard deviation should i include in my fold change or how can i report the fold change results?
In my opinion the first step should be to clarify what kind of inference what do you want to use... a frequentist approach (based on a hypothesis testing) or a probabilistic approach (Bayesian - measuring the strength of statistical evidence)...
FREQUENTIST vs. PROBABILISTIC APPROACHES
Article The evidential statistical paradigm in genetics: STRUG
BAYESIAN APPROACH:
Article Beaumont MA, Rannala B. The Bayesian revolution in genetics....
There are some excel sheets with a formula (based on CT value) available to analyze fold change between 2 groups. You will consider the non-treatment group as control and its value will be fixed as 1. The value of the treatment group will be your fold change in comparison with the control group. You need to calculate SD of the treatment group based on fold change, whereas, SD of the control group will be 0. You can put your CT values in the attached excel sheet and find the fold change.
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