Any research about Scutariellidae in Italy?

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PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Please let me know how to remove mycoplasma contamination from cell line?

I am facing a problem in maintaining the cell line of HepG2 and HepG2.2.15. I have used plasmocin Tm treatment (Invivo gen) Cat.#ant-mpt Lot #MPT-41-01 for more than a month. I have also used...

21 February 2021 6,046 4 View

Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

Can you do Ponceau staining after loading control for Western Blot?

Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...

25 January 2021 757 3 View

What is the correct way to apply a Design effect for sample size calculation when the sampling method is linear systematic random sampling?

When we are doing sampling by means of simple systematic random sampling,how do we calculate the design effect for use during calculation of sample size for our work?

20 January 2021 5,328 3 View

Anybody to help me with Code source for DOUBLE CONCAVE FRICTION PENDULUM BEARINGS Please?

Hello, would you please share the codes for Base Isolation of structures using Double Concave Friction Pendulum Bearings? I am doing a related research but as I am just a beginner in MATLAB, I...

18 January 2021 6,683 1 View

How to generate .dlg files on PyRx?

Hi. I'm using the PyRx version 0.8 and getting the error message: WARNING: Filename mismatch: "2DFN_ADP_CL.maps.fld" :: "..\2DFN_ADP_CL.maps.fld" C:\Program Files (x86)\The Scripps Research...

17 January 2021 843 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Is it possible estimating environmental conditions with a Bayesian approach?

In ecology specific species can be used to get an indication/estimation of the environmental conditions. A simplistic example, lets assume I find a pike (Esox lucius). We know this species does...

01 February 2021 10,034 4 View

How the microplastics levels in freshwater and marine water ecosystem be controlled?

Microplastics have been found in land, air, freshwater, effluent from a wastewater treatment plant and even in tap water and bottled water. The small plastic particles (less than 5 mm) pose a...

19 January 2021 8,637 3 View

Robot that helps clean our seas of microplastics?

My team and I are curently working on a project is focused on preventing microplastics and collecting plastic particles up to 2 * 2mm. Our biggest concern is harming sea life and destroying...

29 November 2020 9,240 5 View

Bisphenol A solubility in water protocol?

Upon some research I found different protocols of BPA solubility, some state a 5 v/v % ethanol (MOTOYAMA et al ) will catalyze the process of solubility and BPA will stay in solution (being an...

06 November 2020 8,152 8 View

Antibiotics removal efficiency in WWTP and Fexcreta?

I am calculating the PEC for selected antibiotic compounds and am having problems finding the readily-available online data about the Fexcreta value for my target compounds (Since the PEC...

02 November 2020 7,675 6 View

Rapid color change in fishes?

I have noted dramatically change in color on the body of some marine fishes. For example, I saw a dramatically change from a "intense-yellow" color to "brown" color (normal color), just in one...

19 October 2020 2,717 6 View

Gas Sensing !! What are the available equipment/tools to find out the composition of gas mixtures ?

We are looking for the equipments/tools to find out the composition of gas mixtures for one of our projects on gas separation. Do you have any idea of how it may impact the following - What...

04 October 2020 2,960 4 View

How to illustrate the occupations of different groups according to environmental gradients?

Hi! I have been working with cave invertebrates from semi-arid regions, and have observed some interesting responses from the community to variables at the local and regional level. I know which...

28 September 2020 1,592 7 View

How nanocatalyst loss can be minimized and effectively immobilized onto glass surface following AOPs in wastewater treatment?

Actually I have faced a significant quantity of nanocatalyst loss during immobilization on glass surface. Though I have used ethanol(volatile) to spread the catalyst effectively towards the...

22 September 2020 4,551 8 View