Hello everyone,
I routinely use cardiac perfusion in rats to fix tissues for IHC/ISH. I use a gravity system and have optimized parameters so I get great results in animals aged PND0-adults. However, I'm going to be doing some experiments where I'm looking at the BBB and really want to make sure that the perfusions are consistent across animals.
Does anyone have a pump system that they really like? It's really important to me that I have really good control over flow rate and there's no bubbles, etc. Extra points if you have one that works well for pups!
Thanks!
Katie
Well probably a little late but I think the Perfusion One system would work really well for rats! We tried it in mice and the volumes were just a little too big to be useful but I think it would be perfect for rats. Money saving tip: you don't really whole electronic system to set the pressure, its very easy to do and maintain by hand.
https://us.vwr.com/store/product/22598894/sacrifice-perfusion-system-leica