Dear fellow researchers,
I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available.
Currently I have thought about the Cysteine assay kit from Sigma. But would like get some recommended methods.
Kind regards,
Raj
The kits depend on how do you plan to use it. (Does it have to be fluorescent?) Maybe you would like to label thiol groups or maybe the carbon atoms. Or maybe as whole. Although the kits are not for general use, it depends on for example how specific you would like to be. If not sigma, promega which is very good. Maybe if it is more biological and not as biochemical, try one more specific.sigma(sigma Aldrich) is a very good one but if not promega. You may need to check the kits available to see if it suits your needs
Yeast could be legally induced to produce an early burst of H2S during winemaking by supplementation with rehydration nutrients that are rich in glutathione. It was proposed that the sulfur-containing amino acid, cysteine, a constituent of glutathione (γ-L-glutamyl-L-cysteinylglycine), is degraded by yeast to generate this early H2S production Yeast has been known since the 1970s to be capable of releasing H2S from cysteine A genome-wide screen of thousands of yeast deletants from yeast single-gene deletion libraries for their H2S production has proven to be a powerful approach to identify yeast genes responsible for H2S formation
he genes identified in this study as affecting H2S formation from cysteine expand not only our current understanding of the cysteine transport process in yeast in a grape juice-like, cysteine-supplemented fermentation condition, but could also be valuable for the breeding of wine yeasts with potential to preserve (enhance) varietal thiols.
Mª Angeles Zorrilla Lopez-Perea thanks for your suggestion. I would like to detect the whole cysteine pool in the cell. It need not to be only fluorescent probe, could be through any other detection method as well.
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