How to change the protein chain names X into A, B, C, D after MD simulations in Gromacs?

I am running MD simulations for holoenzyme in Gromacs. The protein has four chains like A, B, C, D. After MD simulations, All the chains are showing as X chain in VMD and even in the PDB file....

12 November 2020 6,228 1 View

How to filter few markers from a gene set?

I am not good in bioinformatics. I have a gene set which has some 1000 genes which are highly correlating with aging. I want to select some highly significant genes from this geneset for further...

04 November 2020 5,602 5 View

Correlation plot between Gene of Interest and miRNA?

Please suggest portals to retrieve normalized-quantile expressions of micro-RNAs (in RPM).My aim is to construct a correlation plot between the gene of interest and microRNA. I searched in human...

28 October 2020 6,398 3 View

I am trying to do an article on domestic migration. Can anyone suggest me a research gap area where I can do my research on?

We have seen statistically that the domestic migration of workers are increasing every year. People are moving from rural to urban areas for better work and life. This migration has brought in...

03 March 2020 5,445 4 View

How will artificial intelligence help to develop MSMEs ?

Artificial Intelligence is progressive and has wider impact on various sectors. I would like to know how AI is impacting Micro, Small and Medium enterprises. The ways in which it is enacting for...

30 January 2020 7,367 3 View

What will be the perfect markers to differentiate Neural Stem cells from differentiated Neurons?

I've been working on differentiating Rat fetal Neural Stem cells into Neurons with a drug of interest. The Problem here for me is I have used various Neuronal markers like MAP2, TUJ1, NFM to...

15 October 2019 7,114 5 View

Autophagy: After how many days of fasting does autophagy usually start?

Hi, Could you please help me to explore after how many days of fasting does autophagy actually start in (1) Humans (2) Animal models and/or (3) cell lines, please? Thank you in advance

30 June 2019 8,778 5 View

Is there any standarad method or procedure for estimation of water productivity or water use efficiency of integrated farming system?

In integrated farming system (IFS), water applied for one component is also utilized in another component. So how we can compute the WUE and Water productivity of any IFS?

01 April 2019 7,555 2 View

Why the SOD1 mutant model expressing the mutant protein but not the native protein?

I am working with drug activity on familial ALS model cells. I am using mouse Neural stem cells which express the mutated human SOD1 protein. I did western blot with the protein extracted from the...

14 March 2019 511 3 View

Need a help to understand the basic difference in mechanism of antibiotics acting on HeLa and CHO cell lies.?

If we add bacterial strains on HeLa (Cancer) and CHO (normal) cell lines and adding the antimicrobial in order to see the effect of antibiotic inhibiting the cell lines, will it inhibit the cells...

09 May 2018 9,733 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

Can anyone recommend a scale to measure Bloom's taxonomy of learning styles?

I am looking for recommendations of scales (in English or Spanish) to measure learning styles according to Bloom's taxonomy of learning. Thank you in advance!

09 February 2021 1,419 8 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View