09 September 2014 3 1K Report

I need to generate Fab and Fc fragment with Papain and Fab' with pepsin to check the interaction with my ligand. I need to know the protocol for the digestion with papain and pepsin?

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How to determine the dissociation constants and dynamic binding capacity of a matrix for a standard protein which is known to bind?

I want to calculate the dynamic binding capacity and dissociation constants of a gel matrix using a standard protein, anyone has the protocol to calculate the above parameter. Also, at how much...

08 September 2014 388 3 View

Can anybody tell me how to perform molecular dynamics simulation or how to deal with a post translated modified protein struture?

I want to perform molecular dynamics simulation of my protein which is modified for the oxidation of a particular amino acid which has been proved by mass spectrometric analysis. Can any one...

08 September 2014 9,172 7 View

Do we have the option to include dielectric constant while performing a molecular dynamics simulation?

I want to run a simulation of my protein at a particular pH and also wants to include dielectric constant. The dielectric constant  has an impact on binding even if I vary dielectric constant with...

08 September 2014 7,189 6 View

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