My bacterial culture happens to produce extracellular antimicrobial compound. I'm optimizing the conditions for production and I have to determine the rate of production with respect to incubation time. Is there any quantification methods.
GC-FID, GC-MS and HPLC-PDA will help but chemistry expertise is needed
the analytical methodes you choose depends the physical and chemical nature of the antimicrobial compounds. if its polarity is not so low and you have the standards , then a simple filtration, freedry , readded ultra pure water with a simple HPLC-UV-vis injection of the culture and you standard will work.
best regards and good luck
If you have a test for the antimicrobial activity (drop them on E. coli, see if it lives?), you can do a limiting-diultion assay.
Dilute the supernatant containing the compound 1:2, 1:4, 1:8, .... and measure at what dilution it stops killing your target bacteria. The more compound there is, the lower will be the lowest dilution that still kills your target.
Easy, cheap, low-tech and measures directly what you care about.
I have to quantify at the specific time point..the supernatant dilution against pathogens could show whether the compound is more or less but i think it does not show the exact difference in concentration (microgram/ml) with respect to incubation.
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