22 February 2020 4 5K Report

Any mathematical study for sickle cell disease?

More António Maximiano Fernandes's questions See All
¿Are oxygen vacancies in semiconductors capable to change the band gap?

Dear all! Oxygen vacancies do raise negatively the Fermi level by increasing the negative charge in the semiconductor crystal structure. In the case of n-type semiconductors, I wonder if this...

30 July 2024 8,339 2 View

What is the solubility of Iron (+3) in Bismuth Oxide?

Dear all: I am modifying Bismuth Tungstate with Hematite (Fe2O3), so I would like to know about how much Iron(+3) can form a solid solution in a Bismuth Oxide matrix. Best wishes Marco

29 July 2024 2,163 1 View

What should I do if non-specific bands appear only in specific target in western blot?

I tried western blot targeting TTF-1 (Thyroid transcription factor-1) also known as Nkx2.1. I tested two primary antibodies, one is Invitrogen, Nkx2.1 Monoclonal Antibody (8G7G3/1) and the other...

29 May 2024 3,517 3 View

How do I get good bands after PCR of gDNA from bacterial cells?

I have 6 bacterial isolates, I have checked their band on agarose gel electrophoresis, I am getting a genomic band but when I am performing the PCR and after running the gel, I am not getting good...

21 May 2024 8,614 4 View

How to retain the structure after optimization using Gaussian 16?

Dear Experts I have optimized an organic molecule using DFT/B3lYP/ 6-311G using Gaussian16. However, after the optimization, it was found that some bonds were missing from the molecule's...

17 May 2024 9,828 1 View

How to fix " turbulent viscosity limited to viscosity ratio of 1.000000e+05 in xxx cells" ?

Hi everyone, I try to do a simulation about heat transfer of supercritical hydrogen in a cooling channel. Properties of H2 is obtained from NIST and displayed by an UDF function. I used the k-w...

16 May 2024 4,076 2 View

How to express spinors with an arbitrary change of coordinates?

A spinor field in classical differential geometry is defined as a section of a spinor bundle, which is by definition the associated bundle constructed from the spinor representation and the...

14 May 2024 400 0 View

LPS doesn't work. What should I do?

I started to work with C8-D1A cells, mouse astrocyte cell line. We are trying to induce inflammation in cells using LPS, but treatment with LPS does not seem to induce inflammation. I also...

12 May 2024 608 3 View

Would it be proper to mark species that do not migrate as "stationary" or "sedentary" ?

I am curious how should i label species in a certain swamp habitat, since i want to make a difference between the migratory and the "stationary" species. Both of the terms i mentioned in the title...

11 May 2024 3,216 5 View

How can be my research analysis plan?

How do I intend to integrate quantitative and qualitative data analysis techniques to comprehensively examine the intricacies of English and Arabic teaching and learning processes in second...

10 May 2024 1,493 5 View

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Is an invitation to join the editorial board of Clinical Cardiology Updates a scam?

I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...

06 August 2024 8,981 8 View

In the situation below, can I apply braun and Clark thematic analysis?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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