Somebody give me a wheat sample and want to know is it durum wheat or hexaploid wheat (Bread wheat)?. Morphological it seems bread wheat. But how can I confirm it is Bread wheat. Anybody can help me? Thanks
Hi Ashraful,
If you have access to a flow cytometer, you can measure genome size by comparison with a known standard. Hexaploid bread wheat is ~15G bp while tetraploid durum wheat is ~10 Gbp. It is a rapid and reproducible method.
If you don't but have access to a microscope, you can spread root tips on a glass slide and count the chromosome number on metaphases. If you count 42, it's a hexaploid, iand if you count 28 chromosomes it's a durum.
Note that some varieties of bread wheat are octaploids and you can then measure ~40 Gbp/56 chomosomes.
Also check https://www.researchgate.net/publication/227715525_Estimating_Genetic_Diversity_in_Durum_and_Bread_Wheat_Cultivars_from_Turkey_using_AFLP_and_SAMPL_Markers
Hope these following articles will help you.
https://www.mdpi.com/2073-4395/10/1/144/pdf
Article Estimating Genetic Diversity in Durum and Bread Wheat Cultiv...
https://www.frontiersin.org/articles/10.3389/fgene.2019.00717/full
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