How to calculate the CFU/ml for Mycobacterium tuberculosis growing in 7H9 broth?

I am running an experiment in which I need to calculate the CFU/ml of a Mycobacterium tuberculosis growing in 7H9 broth. Is there a reliable method to calculate cfu/ml for MTB since a culture of...

22 January 2021 10,027 2 View

How to import the irradiation flux profile from the ray tracing software TracePro to ANSYS Fluent as a boundary condition for thermal analysis?

I am using TracePro software for optical simulation of my solar concentrator system. The software gives the results as irradiation flux map and also the incident ray table. Now for thermal...

28 March 2020 5,216 4 View

Is Universal Academic Cluster International is a recognized conference?

28 November 2019 4,536 7 View

Can the sign of a coefficient change from + to - when treat for endogeneity?

Hi, I'm running a model to see the growth effects of the exchange rate. The coefficient that I got for the real effective exchange rate is positive significant under the fixed effect model and...

19 November 2019 6,677 1 View

How to interpret the XRD results to find the Chemical composition of the sample under study?

I have used EDX-SEM to identify the elements present in my powdered sample later I have used XRD got the results, now how to interpret the results to find the chemical compounds present in my...

31 October 2019 509 3 View

Is it possible to simulate the electrical output of a silicon PV cell using ANSYS ?

I am working on concentrated photovoltaic thermal system in which i need to simulate both its thermal and electrical output. Using ANSYS I am able to simulate the thermal output but how to do a...

31 October 2019 7,673 2 View

The actual protein band size is around 42 kDa, but a single clear band at around 25 kDa is seen. Is it possible ?

Can such band shifting take place?if not then please suggest some possible causes?

04 December 2018 6,157 5 View

Custom primary antibodies are destroying my samples during immunohistochemistry?

I'm doing IHC to detect dopamine receptors in larval zebrafish sections using custom primary antibodies. I've been following the same protocol I used for all my other IHCs and never have...

06 December 2017 9,114 3 View

PCR doesn't work. DNA extraction by CTAB method?

Hi, I'm extracting bacterial DNA from CTAB/NaCl method. Starting material is either colonies or culture grown in LB broth. I follow the standard method, heat kill, lysozyme+TE 37C 3hrs, then...

05 September 2017 9,289 8 View

What are the techniques used for threshold dose determination of a plant extract in vivo model system?

in albino mice

26 October 2015 2,047 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View