Bacteria have different sensitivity to their or others' autoinducers. When those chemical signals reach a certain centration, the population start to behave in a concerted way, by expressing some proteins for example. What is the threshold concentration? How can we determine it?
Yijie Daniel Deng
If you have standard autoinducer compounds, such as AHLs, you can make a serial dilution of AHLs and then add it to bacterial indicators (such as CV 026 or your working bacteria that get the AHL synthesis gene knock out). You can then determine at what concentration the bacteria start to produce pigment. You can also use realtime PCR to determine when bacteria start to rapidly express functional genes.
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