I have never done CRISPR in zebrafish before. I want to develop a protocol by myself .
I am wondering whether there is a good gene target which can be knocked out and its phenotype can be easily observed?
Thank you for advice!
A good target could be tyrosinase (tyr). You can see the lack of pigment if CRISPR is working well. See this paper for reference: 10.1073/pnas.1308335110
Great. Thanks!
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