Just a proposal to Andrey: you might edit your question text especially 'ICH' to IHC, as well as when choosing the keyword also to use the proposal there: "immunohistochem* ".

This will help to (perhaps) attract more responders especially in the field of immunohistology, immunohistochemistry etc. Also it would be interesting for a serious proposal to know which IHC you would like to perform on which tissue. Thank you in advance, best wishes, and regards, W. H. M.

Good day,

You can try solution of isopropanol, it doesn`t dehydrate and damage cells like ethanol

Dear Andrey,

I agree with Mohamed, and suggest you use either methanol or cold acetone on frozen sections. However, it will also depend on your tissue and your primary antibody of choice. I run the initial comparison test against sections fixed with paraformaldehyde, unless the literature or product sheet say that paraformaldehyde inhibits binding to the epitope. I work with neural tissue; your tissue may be different.

Good Luck!

Jill

Carnoy's solution (60% ethanol, 30% chloroform, 19% acetic acid and 1 g ferric chloride) or Clarke's solution (1;:3 glacial acetic and ethanol) are two fixatives without formalin. You may try

Please read Carnoy's solution composition as 60% ethanol, 30% chloroform, 10% glacial acetic acid and 1 g ferric chloride in my above answer