I am performing ex vivo permeation experiments with a library of different compounds in porcine ocular tissues mounted in Franz-diffusion cells.
For some of the compounds, the plotted permeation profiles (permeated amount vs time) look like the ones in the picture, where there is a noticeable increase in the permeated amount during the first 30 minutes, and then the trend of accumulation decreases. A similar behavior has been reported in several publications but I can't find a clear explanation of what is happening between the first 30 minutes and the rest of the experiment.
Does anyone have a clue about the possible mechanism behind it?
In this data the solubility number increase at the first thirty minutes.
Look up Michaelis-Menten equation. The discussion in the search articles may be helpful.
Dear Laura,
This can be explained via raising the solubility rate during the first 30 minuts. While the solubility increases, the number of molecules in the cell (here Franz-diffusion cell) will become outnumbered. That literally means there would be a saturated level of molecules behind the membrane which cause the high penetration rate through the membrane.
Best
These papers can be useful for lag time and the solubility/permeability relationship in cocrystal/salts.
https://pubs.acs.org/doi/abs/10.1021/acs.cgd.6b00902
https://scholar.google.com/citations?user=FC7RHQ4AAAAJ&hl=en#d=gs_md_cita-d&u=%2Fcitations%3Fview_op%3Dview_citation%26hl%3Den%26user%3DFC7RHQ4AAAAJ%26citation_for_view%3DFC7RHQ4AAAAJ%3AMXK_kJrjxJIC%26tzom%3D-210
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